Coding

Part:BBa_K4258019:Design

Designed by: Jake Schumacher   Group: iGEM22_NDSU   (2022-09-26)


CBDclos-Linker-mScarlet-His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 48
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 48
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 48
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 48
    Illegal NgoMIV site found at 27
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 1076


Design Notes

Bpi1 and Bsa1 sites added to the ends for Golden Gate Cloning.


Source

mScarlet from Addgene Plasmid #121003 Cellulose Binding Domain from BBa_K863111

References